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KMID : 1134820070360020149
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 2 p.149 ~ p.154
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Isolation and Identification of an Antibacterial Substance from Sea Mustard, Undaria pinnatifida, for Streptococcus mutans
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Yun So-Mi
Jang Jun-Ho
Lee Jong-Soo
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Abstract
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An antibacterial substance to the Streptococcus mutans, a causative bacterium for decayed teeth, was isolated from the dried sea mustard, Undaria pinnatifida, and identified by GC and GC/MS. Acetone extract from the sea mustard (10.4 ㎏), was evaporated and partitioned to 4 fractions such as hexane, chloroform, butanol and water. The most active chloroform fraction were further purified through basic alumina, silicic acid and ODS column, successively, and finally, 3 antibacterial substances were isolated on the HPLC attached ODS column by using 95% MeOH and guided with UV detector (254 ㎚). Antibacterial substances (total 160 ㎎, yield 1.5×10?³%) had the same Rf value (0.42) on the TLC developed hexane-diethyl ether?acetic acid (80:30:1) and those methyl esters moved to 0.95. They were identified as the same unsaturated fatty acid, C18:4,n-3 (3,6,9,12-octadecatetraenoic acid, stearidonic acid) compared relative retention times (15.5 min) with authentic fatty acid on the GC chromatogram. It was further confirmed unambiguously on the GC/MS giving molecular ion peak at m/z 290 which coincided with its methyl ester.
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KEYWORD
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sea mustard, Streptococcus mutans, antibacterial activity, stearidonic acid, GC, GC/MS
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